Part:BBa_K3776012
CF3BD
3-hydroxybutyrate dehydrogenase (CF3BD, PDB ID: 5YSS) is a cyclase that catalyzes the cyclization of 5-aminovalerate (5-AVA) to valerolactam. This sequence here is the wild type sequence from Yeom et al. (2018). Its optimal reaction temperature is 40º and does not require any energy source/cofactors.(Gordillo Sierra & Alper, 2020) Our team has performed point mutation modeling on this enzyme to improve its efficiency. Further characterization will be done in the second phase of our project.
Yeom, S. J., Kim, M., Kwon, K. K., Fu, Y., Rha, E., Park, S. H., Lee, H., Kim, H., Lee, D. H., Kim, D. M., & Lee, S. G. (2018). A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-07488-0
Gordillo Sierra, A. R., & Alper, H. S. (2020). Progress in the metabolic engineering of bio-based lactams and their ω-amino acids precursors. Biotechnology Advances, 43, 107587. https://doi.org/10.1016/j.biotechadv.2020.107587
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 266
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |